Rabbit anti-SRSF7 Antibody

• Catalog Number: orb1524206
• Category: Antibodies
• Description: SRSF7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 188 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SRSF7
• UniProt ID: Q16629
• NCBI: NP_001026854.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: serine/arginine-rich splicing factor 7; SR splicin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SRSF7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SRSF7 antibody orb1524206 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SRSF7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SRSF7 antibody orb1524206 used for IP at 6 µg/mg lysate.

Detection of human and mouse SRSF7 by immunohistochemistry.Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-SRSF7 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB