Rabbit anti-SMG9 Antibody

• Catalog Number: orb1527015
• Category: Antibodies
• Description: SMG9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: Between 75 and 125
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A302-210A has not performed satisfactorily when used for WB of SMG9 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of SMG9.; IP - 5 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SMG9
• UniProt ID: Q9H0W8
• NCBI: NP_061981.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: protein smg-9 homolog; Protein SMG-9; protein SMG9
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SMG9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMG9 antibody orb1527015 used for IP at 10 µg/mg lysate.

Detection of human SMG9 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SMG9 (Cat. No. orb1527015) used at a dilution of 1: 5, 000 (0.2 µg/ml). Detection: DAB