Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody

• Catalog Number: orb1523050
• Category: Antibodies
• Description: RNA Polymerase II, Phospho (S5) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Surrounding serine 5
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:400. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RNA Polymerase II, Phospho (S5)
• UniProt ID: P24928
• NCBI: NP_000928.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: RPBh1; RPOL2; RpIILS; RPB1; RNA-directed RNA polym
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho RNA Polymerase II (S5) by western blot. Samples: Whole cell lysate (2 µg) from HeLa cells prepared using NETN lysis buffer. Lysates were either mock treated (-) or treated with phosphatases (+). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (orb1523050) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-RNA Polymerase II antibody.

Detection of human Phospho RNA Polymerase II (S5) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 1% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Immunoprecipitates were either mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (orb1523050) used for IP at 6 µg per reaction.

Detection of human Phospho RNA Polymerase II (S5) by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) (Cat. No. orb1523050) used at a dilution of 1: 200 (0.2µg/ml). Detection: DAB

Detection of mouse Phospho RNA Polymerase II (S5) by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) (Cat. No. orb1523050) used at a dilution of 1: 200 (0.2µg/ml). Detection: DAB