Rabbit anti-Phospho MCM2 (S108) Antibody

• Catalog Number: orb1530699
• Category: Antibodies
• Description: MCM2, Phospho (S108) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Surrounding serine 108
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:5,000 - 1:10,000; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MCM2, Phospho (S108)
• UniProt ID: P49736
• NCBI: NP_004517.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: D3S3194; MITOTIN; minichromosome maintenance prote
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse Phospho MCM2 (Ser108) by western blot.Samples: Whole cell lysate from NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser108) antibody at 1 µg/ml. Lower Panel: Rabbit anti-MCM2 antibody.

Detection of human Phospho MCM2 (Ser108) by western blot.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser108) antibody at 0.1 µg/ml.

Detection of human Phospho MCM2 (S108) by immunohistochemistry. Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of mouse Phospho MCM2 (S108) by immunohistochemistry. Sample: FFPE sections of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human Phospho MCM2 (Ser108) by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells treated with etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-MCM2 antibody.