PD-L1 Rabbit Recombinant Monoclonal Antibody

SKU: orb1519952

Description

PD-L1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells. ICC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Reactivity: Human
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR020E
• Immunogen: Between 240 and C-terminus
• Target: PD-L1
• Purity: Recombinant antibody was purified from cell culture supernatant
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7 homolog 1; B7-H; B7H1; B7-H1; CD antigen CD274; CD274 antigen; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; programmed cell death 1 ligand 1; Programmed death ligand 1

Quick Database Links

Gene Symbol

PD-L1

UniProt

Q9NZQ7

RefSeq (Protein):NP_054862.1