Rabbit anti-PD-L1 Recombinant Monoclonal Antibody

SKU: orb1519952

Description

PD-L1 Antibody

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells. ICC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Reactivity: Human
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR020E
• Immunogen: Between 240 and C-terminus
• Target: PD-L1
• Purity: Recombinant antibody was purified from cell culture supernatant

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Disclaimer: For research use only

Alternative Names

PDCD1L1; Programmed death ligand 1; programmed cell death 1 ligand 1; PD-L1; PDL1; PDCD1LG1; PDCD1 ligand 1; CD274 antigen; CD antigen CD274; B7-H1; B7H1; B7 homolog 1; B7-H

Detection of human PD-L1 by western blot. Samples: Whole cell lysate (50 µg) from RKO, Jurkat, SUP-M2, HEK293T, and HDLM-2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody

Detection of human PD-L1 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human PD-L1 by immunocytochemistry. Sample: FFPE section of human SUP-M2 cells. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human PD-L1 (red) and Lamin-A/C (green) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-PD-L1 recombinant monoclonal and rabbit anti-Lamin-A/C. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG and DyLight® 488-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).

Detection of human PD-L1 (green) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Detection of human PD-L1 by immunocytochemistry. Sample: FFPE section of human SUP-M2 cells. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody Secondary: HRP-conjugated goat anti-rabbit IgG

Quick Database Links

Gene Symbol

PD-L1

UniProt

Q9NZQ7

RefSeq (Protein):NP_054862.1