Rabbit PCAF Antibody

SKU: orb1527995

Description

PCAF Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ChIP, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 782 and C-terminus
• Target: PCAF
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAF; CREBBP-associated factor; histone acetylase PCAF; histone acetyltransferase KAT2B; histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/CAF; p300/CBP-associated factor; PCAF

Detection of human and mouse PCAF by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCAF antibody orb1527995 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PCAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCAF antibody orb1527995 used for IP at 6 µg per reaction.

Localization of PCAF Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-PCAF antibody orb1527995 and analyzed by DNA sequencing. The figure illustrates the peak distribution of PCAF binding within a 650 Kb region of chromosome 1 as detected using anti-PCAF antibody orb1527995.

Quick Database Links

Gene Symbol

PCAF

UniProt

Q92831

RefSeq (Protein):NP_003875.3