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Catalog Number | orb1530567 |
---|---|
Category | Antibodies |
Description | MCM3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 500 and 550 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate. Use MCM2 antibody A300-191A or MCM5 antibody A300-195A to IP the MCM complex.; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MCM3 |
UniProt ID | P25205 |
NCBI | NP_002379.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | p102; RLFB; RLF subunit beta; P1-MCM3; P1.h; minic Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse MCM3 by western blot.Samples: Whole cell lysate from HeLa, Jurkat, HEK293T, and TCMK-1 cells. Antibody: Affinity purified rabbit anti-MCM3 antibody orb1530567 at 0.04 µg/ml.
Detection of human MCM3 by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human MCM3 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-MCM3 antibody.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |