Rabbit anti-IL-4 Recombinant Monoclonal Antibody

• Catalog Number: orb1806422
• Category: Antibodies
• Description: Rabbit anti-IL-4 Recombinant Monoclonal Antibody
• Target: IL-4
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 100 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Residues 25-153
• UniProt ID: P05112
• Tested applications: IP, WB
• Dilution range: WB: 1:1000, IP: 20 µl/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR298L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BSF1; interleukin 4 variant 2; pitrakinra; interle
• Note: For research use only

Detection of human IL-4 by western blot. Samples: Whole cell lysate (10 µg) from 293-F untransfected and IL-4 overexpressing (hOE) cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IL-4 recombinant monoclonal antibody (orb1806422) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human IL-4 by western blot. Samples: Human recombinant IL-4 (0.1 µg) protein. Antibody: Rabbit anti-IL-4 recombinant monoclonal antibody (orb1806422) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human IL-4 by western blot of immunoprecipitates. Samples: Whole cell lysate (500 µg per IP; 5% of IP loaded) from 293F cells overexpressing human IL-4. Antibodies: Rabbit anti-IL-4 recombinant monoclonal antibody (orb1806422) used for IP at 20 µl/mg lysate.