Rabbit anti-HDGF Antibody

• Catalog Number: orb1525331
• Category: Antibodies
• Description: HDGF Antibody
• Target: HDGF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 125 and 175
• UniProt ID: P51858
• Tested applications: IHC, IP
• Dilution range: WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: high mobility group protein 1-like 2; epididymis s
• Note: For research use only
• NCBI: NP_004485.1

Detection of human HDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDGF antibody orb1525331 used for IP at 6 µg/mg lysate.

Detection of mouse HDGF by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF used at a dilution of 1: 1, 000 (1µg/ml) or 1: 200 (1µg/ml). Detection: DAB

Detection of human HDGF by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HDGF used at a dilution of 1: 1, 000 (1µg/ml) or 1: 200 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).