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Item 1 of 4
Item 1 of 4
Rabbit anti-GFP Recombinant Monoclonal Antibody
Catalog Number: orb1784590
| Catalog Number | orb1784590 |
|---|---|
| Category | Antibodies |
| Description | Rabbit anti-GFP Recombinant Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | BLRE04L |
| Tested applications | ICC, IP, WB |
| Immunogen | Green Fluorescent Protein |
| Antibody Type | Primary Antibody |
| Concentration | 50 µg/ml |
| Dilution range | WB: 1:1000, IP: 20 µl/mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | GFP |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of GFP by western blot.Samples: Whole cell lysate from HEK293T cells transfected with EGFP-KAP1, KAP1-EGFP, or untransfected. Antibody: Rabbit anti-GFP recombinant monoclonal antibody used at 1:1000.
Detection of GFP by western blot.Samples: Whole cell lysate from 293T cells transfected with EGFP, YP, CFP, AcGFP, or BFP. Antibody: Rabbit anti-GFP recombinant monoclonal antibody used at 1:1000.
Detection of GFP by western blot of immunoprecipitates.Samples: Whole cell lysate from 293-F cells tranfected with EGFP-KAP1. Antibodies: Rabbit anti-GFP recombinant monoclonal antibody.
Detection of GFP by ICC. Sample: FFPE section of HEK293 cells expressing a GFP nuclear protein (left) and untransfected HEK293 cells (right). Antibody: Rabbit anti-GFP recombinant monoclonal antibody.
