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Rabbit anti-GAK Antibody
Catalog Number: orb1523309
| Catalog Number | orb1523309 |
|---|---|
| Category | Antibodies |
| Description | GAK Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 700 and 750 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | GAK |
| UniProt ID | O14976 |
| NCBI | NP_005246.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | auxilin-2; cyclin-G-associated kinase; DNAJ26; DNA Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of mouse GAK by western blot. Samples: Whole cell lysate (10 µg) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody orb1523309 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human GAK by western blot. Samples: Whole cell lysate (10 µg) from GaMG, HeLa, HEK293T, Jurkat, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody orb1523309 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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