Rabbit anti-G3BP1 Antibody

• Catalog Number: orb1527354
• Category: Antibodies
• Description: G3BP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 275 and 325
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: G3BP1
• UniProt ID: Q13283
• NCBI: NP_005745.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: GAP SH3 domain-binding protein 1; Ras-GTPase-activ
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse G3BP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1 antibody orb1527354 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human G3BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G3BP1 antibody orb1527354 used for IP at 6 µg per reaction.