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Catalog Number | orb1527898 |
---|---|
Category | Antibodies |
Description | FOXK1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 683 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | FOXK1 |
UniProt ID | P85037 |
NCBI | NP_001032242.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | forkhead box protein K1; FOXK1L; MNF; myocyte nucl Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human FOXK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-FOXK1 antibody orb1527898 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. FoxK1 was also immunoprecipitated by rabbit anti-FOXK1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human and mouse FOXK1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FOXK1 (Cat. No. orb1527898) used at a dilution of 1: 1, 000 (0.2µg/ml) and 1: 200 (1µg/ml). Detection: DAB
IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |