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Catalog Number | orb1527960 |
---|---|
Category | Antibodies |
Description | ESCO2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 200 and 250 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | ESCO2 |
UniProt ID | Q56NI9 |
NCBI | NP_001017420.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | ECO1 homolog 2; RBS; N-acetyltransferase ESCO2; Es Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human ESCO2 by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody (orb1527960) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human ESCO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 15 % of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ESCO2 antibody (orb1527960) used for IP at 6 µg per reaction. ESCO2 was also immunoprecipitated by a previous lot of this antibody (orb1527960) and a second antibody against a different epitope of ESCO2.