Rabbit anti-EAF2 Antibody

• Catalog Number: orb1526505
• Category: Antibodies
• Description: EAF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: Between 210 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A302-503A has not performed satisfactorily when used for WB of EAF2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of EAF2.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EAF2
• UniProt ID: Q96CJ1
• NCBI: NP_060926.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BM040; ELL-associated factor 2; testosterone-regul
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EAF2 antibody orb1526505 used for IP at 3 µg/mg lysate.

Detection of human EAF2 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-EAF2 (Cat. No. orb1526505) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB