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Catalog Number | orb1088200 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to dsDNA(anti-double stranded DNA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to dsDNA(anti-double stranded DNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain dsDNA(anti-double stranded DNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of dsDNA(anti-double stranded DNA) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Rabbit |
Range | 0.32-20 ng/mL |
Sensitivity | 0.098ng/mL |
Protein Length | 3.5h |
Alternative names | dsDNA Read more... |
Note | For research use only |
Sample Types | serum, plasma, urine and other biological fluids |
Expiration Date | Please enquire. |
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