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Rabbit anti-DNL3 Antibody
Catalog Number: orb1528051
| Catalog Number | orb1528051 |
|---|---|
| Category | Antibodies |
| Description | DNL3 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 900 and 950 |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | DNL3 |
| UniProt ID | P49916 |
| NCBI | CAA59230.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | DNA ligase III; ligase III, DNA, ATP-dependent; po Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human DNL3 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody (orb1528051) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human DNL3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNL3 antibody (orb1528051) used for IP at 6 µg per reaction. DNL3 was also immunoprecipitated by a previous lot of this antibody (orb1528051) and a second antibody against a different epitope of DNL3.
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