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Rabbit anti-CARD9 Antibody
Catalog Number: orb1520262
| Catalog Number | orb1520262 |
|---|---|
| Category | Antibodies |
| Description | CARD9 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 450 and 500 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | CARD9 |
| UniProt ID | Q9H257 |
| NCBI | Q9H257.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | hCARD9; CANDF2; caspase recruitment domain-contain Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human CARD9 by western blot. Samples: Whole cell lysate (50 µg) from SW620 and HT-29 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARD9 antibody (orb1520262) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CARD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARD9 antibody (orb1520262) used for IP at 1 µg per reaction.
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