Rabbit anti-CAR Antibody

• Catalog Number: orb1525886
• Category: Antibodies
• Description: CAR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 315 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CAR
• UniProt ID: P78310
• NCBI: NP_001329.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: CAR; HCAR; CVB3-binding protein; coxsackievirus B-
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CAR by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CAR antibody orb1525886 used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. CAR was also immunoprecipitated by rabbit anti-CAR antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human CAR by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAR (Cat. No. orb1525886) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate