Rabbit anti-BubR1 Antibody

• Catalog Number: orb1529253
• Category: Antibodies
• Description: BubR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BubR1
• UniProt ID: O60566
• NCBI: NP_001202.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: MAD3L; Protein SSK1; SSK1; MVA1; mitotic checkpoin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BubR1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BubR1 used at a dilution of 1:200 (1µg/ml).

Detection of human BubR1 by WB and IP.Samples: HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody orb1529253 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).