RAB7/RAB7A Antibody

• Catalog Number: orb334616
• Category: Antibodies
• Description: RAB7/RAB7A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Predicted Reactivity: Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB7 (157-196aa KEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKND), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 23490 MW
• UniProt ID: P51149
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ras-related protein Rab-7a;RAB7A;RAB7;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-RAB7 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-RAB7 antibody.1:human HeLa cell;2:human A375 cell;3:huamn A431 cell;4:human HL-60 cell;5:human U87 cell; 6:human MCF-7 cell;7:human THP-1 cell;8:rat brain tissue;9:rat lung tissue;10:mouse brain tissue;11:mouse lung tissue.

IF analysis of RAB7 using anti-RAB7 antibody. RAB7 was detected in immunocytochemical section of U20S cells.

IF analysis of RAB7 using anti-RAB7 antibody. RAB7 was detected in immunocytochemical section of MCF7 cells.