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Catalog Number | orb527052 |
---|---|
Category | Antibodies |
Description | Rab5/RAB5A Antibody (monoclonal, 3E9) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | Clone: 3E9 |
Tested applications | FC, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG2b |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 24 kDa |
UniProt ID | P20339 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Ras-related protein Rab-5A; RAB5A; RAB5 Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-Rab5/RAB5A antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis using anti-Rab5/RAB5A antibody.Lane 1:HeLa cell;2:human placenta tissue;3:A549 cell.4:A375 cell;5:human SW620 cell;6:human U20S cell;7:human HEK293 cell;8:human U87 cell;9:rat brain tissue;10:mouse brain tissue;11:mouse Neuro-2a cell.
IF analysis of RAB5A using anti-RAB5A antibody. RAB5A was detected in immunocytochemical section of MCF7 cells.
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