Rab5/RAB5A Antibody (monoclonal, 3E9)

• Catalog Number: orb527052
• Category: Antibodies
• Description: Rab5/RAB5A Antibody (monoclonal, 3E9)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Clone: 3E9
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 24 kDa
• UniProt ID: P20339
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ras-related protein Rab-5A; RAB5A; RAB5
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Rab5/RAB5A antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-Rab5/RAB5A antibody.Lane 1:HeLa cell;2:human placenta tissue;3:A549 cell.4:A375 cell;5:human SW620 cell;6:human U20S cell;7:human HEK293 cell;8:human U87 cell;9:rat brain tissue;10:mouse brain tissue;11:mouse Neuro-2a cell.

IF analysis of RAB5A using anti-RAB5A antibody. RAB5A was detected in immunocytochemical section of MCF7 cells.