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Catalog Number | orb2292288 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant RAB3B. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3F12 |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2a Kappa |
Immunogen | RAB3B (AAH05035, 120 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | IKTYSWDNAQVILVGNKCDMEEERVVPTEKGQLLAEQLGFDFFEASAKENISVRQAFERLVDAICDKMSDSLDTDPSMLGSSKNTRLSDTPPLLQQNCSC |
NCBI | AAH05035 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged RAB3B is approximately 0.1 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to RAB3B on HeLa cell. [antibody concentration 10 ug/ml]
Immunoperoxidase of monoclonal antibody to RAB3B on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
RAB3B monoclonal antibody (M01), clone 3F12. Western Blot analysis of RAB3B expression in IMR-32.
Western Blot analysis of RAB3B expression in transfected 293T cell line by RAB3B monoclonal antibody (M01), clone 3F12. Lane 1: RAB3B transfected lysate (24.758 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (36.63 KDa).