RAB18 antibody

• Catalog Number: orb1473664
• Category: Antibodies
• Description: Mouse monoclonal antibody to RAB18
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human RAB18. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RAB18
• Entrez: 19330, 307039, 22931
• UniProt ID: Q5EB77, P35293, Q9NP72
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Ras-related protein Rab-18
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RAB18 expression in PC3 (A), mouse brain (B), rat brain (C), human brain (D), human testis (E) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 23 kD)

Flow cytometric analysis of PC3 cells using Anti-RAB18 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.