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Catalog Number | orb623777 |
---|---|
Category | Antibodies |
Description | RAB11B Antibody (monoclonal, 6C5) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 6C5 |
Tested applications | FC, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG2b |
Immunogen | A synthetic peptide corresponding to a sequence of human RAB11B (QKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 24 kDa |
UniProt ID | Q15907 |
Sensitivity | > 5000 cells |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Ras-related protein Rab-11B;GTP-binding protein YP Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Expiration Date | 12 months from date of receipt. |
IF analysis of RAB11B using anti-RAB11B antibody.
Overlay histogram showing Caco-2 cells stained with M04526 (Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.
WB analysis using anti-RAB11B antibody.Lane 1:HEK293 tissue, Lane 2:HeLa cell, Lane 3:A549 cell, Lane 4:placenta cell, Lane 5:HepG2 cell, Lane 6:Caco-2 cell, Lane 7:THP-1 cell, Lane 8:Raji cell.
WB analysis using anti-RAB11B antibody.1:rat brain tissue, 2:rat lung cell, 3:rat spleen cell, 4:rat C6 cell, 5:mouse brain cell.6:mouse lung cell.7:mouse spleen cell.8:mouse Neuro-2a cell.9:mouse RAW246.7 cell.
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