R-Cadherin-4 CDH4-Antibody

• Catalog Number: orb546377
• Category: Antibodies
• Description: R-Cadherin-4 CDH4-Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human R Cadherin/CDH4 recombinant protein (Position: K458-D641).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 125 kDa
• UniProt ID: P55283
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cadherin-4; Retinal cadherin; R-CAD; R-cadherin; C
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-R Cadherin/CDH4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of R Cadherin/CDH4 using anti-R Cadherin/CDH4 antibody.Lane 1:human U20S cell; 2:human U87 cell; 3:human A549 cell; 4:human CACO-2 cell; 5:human HepG2 cell; 6:human K562 cell; 7:human HELA cell.

IHC analysis of R Cadherin/CDH4 using anti-R Cadherin/CDH4 antibody. R Cadherin/CDH4 was detected in paraffin-embedded section of human glioma tissue.