QKI (pan) Antibody

• Catalog Number: orb150946
• Category: Antibodies
• Description: Mouse Anti-Human QKI (pan) Monoclonal IgG2b
• Target: QKI
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Fusion protein amino acids 1-341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) > 90% identity with QKI-6, QKI-7 and QKI-7b.
• UniProt ID: Q96PU8
• MW: 36-38kDa
• Tested applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000)
• Application notes: 1 µg/ml of SMC-467 was sufficient for detection of Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N147/6 (Formerly sold as S147-6)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Protein quaking Antibody, Hqk Antibody, HqkI Antib
• Research Area: Neuroscience, Protein Biochemistry, Signal Transdu
• Note: For research use only
• Entrez: 9444
• NCBI: NP_006766.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI (pan) Monoclonal Antibody, Clone N147/6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI (pan) Antibody (D) Composite.