Pyruvate Kinase (PKM2) Antibody (C-term)

• Catalog Number: orb1788119
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: 476-505 aa
• Dilution range: WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 57937 Da
• Target: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
• UniProt ID: P14618
• NCBI: NP_001193728.1, NP_001193725.1, NP_002645.3, NP_872270.1, NP_872271.1, NP_001193727.1, NP_001193726.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate (from left to right), using PKM2-N491. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

All lanes: Anti-PKM2 Antibody N491 at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: mouse brain lysates. Lane 3: NIH/3T3 whole cell lysates. Lane 4: PC-12 whole cell lysates. Lane 5: Ramos whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57.9 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.