pyruvate kinase L/R Antibody

• Catalog Number: orb556755
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Pyruvate Kinase (liver/RBC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 62
• Target: Pyruvate Kinase (liver/RBC)
• UniProt ID: P30613
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: pyruvate kinase L/R , PK1 , PKL , PKRL , RPK
• Note: For research use only
• Application notes: Positive control: 293T , A431 , HeLa , HepG2 , A375 , Rat liver , PKLR-transfected 293T
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using Pyruvate Kinase (liver/RBC) antibody

Immunohistochemical staining of rat liver using Pyruvate Kinase (liver/RBC) antibody

Immunohistochemical staining of NCIN87 xenograft using Pyruvate Kinase (liver/RBC) antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2), HeLa(Lane 3), HepG2(Lane 4), A375(Lane 5) using Pyruvate Kinase (liver/RBC) antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2), HeLa(Lane 3), HepG2(Lane 4), A375(Lane 5) using Pyruvate Kinase (liver/RBC) antibody