Pyrophosphatase 1/PPA1 Antibody

• Catalog Number: orb546376
• Category: Antibodies
• Description: Pyrophosphatase 1/PPA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Pyrophosphatase 1/PPA1 recombinant protein (Position: E7-N236).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 33 kDa
• UniProt ID: Q15181
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Inorganic pyrophosphatase; Pyrophosphate phospho-h
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of PPA1 using anti-PPA1 antibody.Lane 1:human U937 cell; 2:human CACO-2 cell; 3:human Raji cell; 4:human PC-3 cell; 5:human HEK293 cell; 6:human HL-60 cell; 7:human K562 cell; 8:rat liver tissue; 9:mouse liver tissue.

IHC analysis of PPA1 using anti-PPA1 antibody.PPA1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of PPA1 using anti-PPA1 antibody.PPA1 was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of PPA1 using anti-PPA1 antibody.PPA1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of PPA1 using anti-PPA1 antibody.PPA1 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of PPA1 using anti-PPA1 antibody.PPA1 was detected in paraffin-embedded section of rat brain tissue.