Pyronine B

SKU: orb2901542

Description

Pyronin B is an organic cationic dye used for the staining of bacteria, mycobacteria and ribonucleic acids. Pyronin B is also used as a small hydrophobic (SH) protein channel inhibitor.

Key Properties

• CAS Number: 2150-48-3
• MW: 358.91
• Purity: >98% (HPLC)
• Formula: C₂₁H₂₇ClN₂O
• SMILES: [Cl-].[O+]=1C=2C=C(C=CC2C=C3C=CC(=CC13)N(CC)CC)N(CC)CC
• Target: Autophagy
• Solubility: In Vitro: DMSO : 20.83 mg/mL (58.04 mM; Ultrasonic (<60°C)

Bioactivity

• In Vitro:
  Pyronin B (0-15 μM) inhibits channel activity of approximately 60% of small hydrophobic (SH) proteins at a concentration of 10 μM, and the Kd value of 6.8 μM. Pyronin B (0-0.25 μM) affects RSV replication in Vero cells in a dose-dependent manner, and its TCID50 (50% tissue culture infection dose) = 0 at the concentration of 0.25 μM. Pyronin B (10 μM) can react with bromate in an oxidation reaction and the reaction rate increases dramatically with the addition of trace amounts of Ti (IV) with the characteristic absorption spectra of 555 nm.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Autophagy