PXN Antibody

• Catalog Number: orb1672020
• Category: Antibodies
• Description: PXN Antibody
• Clonality: Recombinant
• Clone Number: LD2 sAB
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: Peptide corresponding to paxillin-LD2 motif (residues 141-156) fused to MBP; peptide sequence: NLSELDRLLLELNAVQHNP.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: PXN
• UniProt ID: P49023
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PXN
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-Paxillin (LD2 Motif) antibody LD2 s97. HepG2 (A)- A431 (B)- and HeLa (C) cell lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of LD2 s2 at 2 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Paxillin is 64.5 kDa. orb1672020 successfully detected Paxillin in HepG2- A431- and HeLa cell lysate samples.