Pvr Antibody

• Catalog Number: orb1671696
• Category: Antibodies
• Description: Pvr Antibody
• Clonality: Recombinant
• Clone Number: 3F1
• Tested applications: ELISA, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising rats with recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Pvr
• UniProt ID: Q8K094
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TAGE 3F1, Poliovirus receptor, cellular receptor f
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-CD155 antibody 3F1. Mouse thymus lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric r version of 3F1 (orb1671696) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of RAW 264.7 cells using anti-CD155 antibody (3F1). Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric r version of 3F1 (orb1671696) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671696- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Binding curve of anti-CD155 antibody 3F1 (orb1671696) to recombinant mouse CD155 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse CD155 Fc-Fusion Protein (Pr00231-1.9; Antibody) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 ng/ml was performed using orb1671696. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.