PVALB Antibody

• Catalog Number: orb1247143
• Category: Antibodies
• Description: PVALB Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-GVDEFSTLVAES
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1). Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3ug/ml. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PVALB
• UniProt ID: P20472
• NCBI: NP_002845.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumi
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1). Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3ug/ml. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247143 (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer).r. Detected by chemiluminescence.

orb1247143 (0.15 ug/ml) staining of adenovirus-mediated gene transfer in Rat Heart cells 1) Untransfected, 2) transient transfection with Human PVALB. 3) Untransfected Rat Skeletal Muscle. Detected by chemiluminescence.

orb1247143 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247143 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).