Purmorphamine

SKU: orb1307399

Description

Purmorphamine is a potent Smoothened (Smo) agonist that competitively inhibits BODIPY-cyclopamine binding. It is widely used in Hedgehog pathway research to induce osteogenic differentiation in stem cells, applicable in both in vitro and in vivo bone biology studies.

Research Area

Cell Biology, Pharmacology & Drug Discovery, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 483367-10-8
• MW: 520.62
• Purity: 99.48% (May vary between batches)
• Formula: C₃₁H₃₂N₆O₂
• SMILES: C1CCC(CC1)n1cnc2c(Nc3ccc(cc3)N3CCOCC3)nc(Oc3cccc4ccccc34)nc12
• Target: Smo,Autophagy,Hedgehog/Smoothened
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.84 mM);DMSO:31 mg/mL (59.54 mM)

Bioactivity

• Target IC50:
  Smo:1.5 μM
• In Vivo:
  In multipotent C3H10T1/2 cells, Purmorphamine can induce osteogenesis with an EC50 of 1 μM. It directly binds to Smoothened, thereby activating the Hedgehog pathway with an IC50 of 1.5 μM.
• In Vitro:
  In rat models, based on human mesenchymal stem cells, Purmorphamine upregulates the expression of ALP.
• Cell Research:
  C3H10T1/2 cells are expanded in T175 flasks; cells at 13th passage are detached by trypsin/EDTA and diluted in the growth media. The resulting cell suspension is then plated into black clear bottom 384-well plates with 2500 cells/well in 100 μL growth medium using a Multi-dropTM liquid delivery system. After overnight incubation, cells attached to the bottom of the wells. A stock solution of each Purmorphamine in DMSO (500 nL) is delivered into corresponding well using a Mini TrakTM multiposition dispenser system to make a final concentration of 5 μM of Purmorphamine. Cells are then incubated at 37 ℃ with 5% CO2 in air atmosphere. After 4 days, the medium is removed and 10 μL of passive lysis buffer is added into each well. After 5 min, 10 μL of alkaline phosphatase substrate solution is added to each well. After incubating 15 min at room temperature, the plates are read on an Acquest high-throughput plate reader following the manufacturer's protocol.(Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Autophagy, inhibit, Hedgehog, Inhibitor, Shh Signaling Antagonist VI, Purmorphamine, Smoothened, Smo

Compound Identifiers

PubChem CID

5284329