PUM1 antibody

• Catalog Number: orb340996
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PUM1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Pumilio 1
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:50-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PUM1
• Entrez: 80912, 9698
• UniProt ID: Q80U78, Q14671
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: KIAA0099; PUMH1; Pumilio homolog 1; HsPUM; Pumilio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Pumilio 1 expression in K562 (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 119; 124; 126 kD; Observed band size: 135 kD)

Immunofluorescent analysis of Pumilio 1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).