PtX His Tag (C-term) (3D5) Recombinant Antibody (HRP)

SKU: orb1736839

Description

Horseradish peroxidase (HRP) conjugated recombinant monoclonal antibody against C-terminal polyhistidine tagged proteins. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: Suggested dilution is 1: 500 - 1: 5 000 for WB and 1: 1 000 - 1: 150 000 for ELISA. Optimal dilutions/concentrations should be determined by the user.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Plant
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3D5
• Target: Polyhistidine tag/ purified his-tagged Fab fragment at the carboxy-terminal
• Source/Expression System: N. Benthamiana plants
• Molecular Weight: ± 150 kDa
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Conjugation: HRP

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.1 M Phosphate Buffered Saline (PBS), 25% glycerol preservative, pH = 7.4
• Concentration: 0.5 mg/ml
• Hazard Information: Non-Hazardous
• Disclaimer: For research use only

Alternative Names

polyhis tag antibody, anti-his, 6xhis tag antibody, histidine antibody, his tag, his-tagged protein

Quick Database Links

Gene Symbol

Polyhistidine tag/ purified his-tagged Fab fragment at the carboxy-terminal

UniProt

NA