PTPRC (phospho-Y1216) antibody

• Catalog Number: orb515266
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PTPRC (phospho-Y1216)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1216 of human CD45 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PTPRC
• Entrez: 24699, 5788
• UniProt ID: P04157, P06800, P08575
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: CD45; Receptor-type tyrosine-protein phosphatase C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD45 (Phospho-Y1216) expression in HEK293T (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates. (Predicted band size: 147 kD; Observed band size: 180 kD)

Direct ELISA antibody dose-response curve using Anti-CD45 (Phospho-Y1216) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.