PTP1B/PTPN1 Antibody

• Catalog Number: orb402385
• Category: Antibodies
• Description: PTP1B/PTPN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PTP1B (4-30aa EKEFEQIDKSGSWAAIYQDIRHEASDF), different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 49967 MW
• UniProt ID: P18031
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tyrosine-protein phosphatase non-receptor type 1;3
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-PTP1B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of PTP1B using anti-PTP1B antibody.Lane 1:rat kidney Tissue;2:mouse kidney Tissue.

WB analysis of PTP1B using anti-PTP1B antibody.Lane 1:human placenta tissue;2:human MCF-7 cell;3:human Caco-2 cell;4:human K562 cell.5:human U2O cell;6:human PC-3 cell;7:human HL-60 cell;8:human A549 cell.

IF analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in paraffin-embedded section of mouse spleen tissues.

IHC analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in paraffin-embedded section of mouse testis tissues.

IHC analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in paraffin-embedded section of rat spleen tissues.

IHC analysis of PTP1B using anti-PTP1B antibody.PTP1B was detected in frozen section of human placenta tissues.