PTK6 (phospho-Y447) antibody

• Catalog Number: orb515495
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PTK6 (phospho-Y447)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y447 of human BRK protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PTK6
• Entrez: 5753
• UniProt ID: Q13882
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: BRK; Protein-tyrosine kinase 6; Breast tumor kinas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BRK (Phospho-Y447) expression in HEK293T (A), rat kidney (B), rat lung (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52 kD)

Immunofluorescent analysis of BRK (Phospho-Y447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.