PTK2 Antibody

• Catalog Number: orb1261299
• Category: Antibodies
• Description: PTK2 Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: [E1E5]
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human FAK
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 102kDa
• Target: PTK2
• UniProt ID: Q05397
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FA
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FAK antibody (orb1261299). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse spleen using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human spleen using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of SH-SY-5Y cells using FAK antibody (orb1261299).

Immunofluorescence analysis of PANC-1 cells using FAK antibody (orb1261299).