PTGS1 Antibody

• Catalog Number: orb1247911
• Category: Antibodies
• Description: PTGS1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-QDDGPAVERPSTEL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa and 70-75kDa bands observed in lysates of cell line U937 and approx 70-75kDa band observed in Mouse Kidney and Skeletal Muscle lysates (calculated MW of 64.5kDa according to Human NP_542158.1 and 68.7kDa Human NP_000953.2, and 69.0kDa according to Mouse NP_032995.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus and vesicles of NIH3T3 cells. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PTGS1
• UniProt ID: P23219
• NCBI: NP_000953.2, NP_542158.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa and 70-75kDa bands observed in lysates of cell line U937 and approx 70-75kDa band observed in Mouse Kidney and Skeletal Muscle lysates (calculated MW of 64.5kDa according to Human NP_542158.1 and 68.7kDa Human NP_000953.2, and 69.0kDa according to Mouse NP_032995.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus and vesicles of NIH3T3 cells. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247911 (2 ug/ml) staining of U937 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247911 (3.8 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247911 (0.5 ug/ml) staining of Mouse Kidney (A) and Skeletal Muscle (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247911 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi and vesicle staining.