PTBP1 Antibody

• Catalog Number: orb1247059
• Category: Antibodies
• Description: PTBP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: DGIVPDIAVGTKR-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human, Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat, HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.01-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver and Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration: 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PTBP1
• UniProt ID: P17225
• NCBI: NP_002810.1, NP_114368.1, NP_787041.1, NP_114367.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PTBP1, PTB, polypyrimidine tract binding protein 1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human, Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat, HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.01-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver and Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration: 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247059 (0.1 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247059 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

orb1247059 (0.01 ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247059 (0.03 ug/ml) staining of lysates of cell line NIH3T3 (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247059 (0.1-0.3 ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.