PSMD1 Antibody

• Catalog Number: orb1524061
• Category: Antibodies
• Description: PSMD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 903 and 953
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PSMD1
• UniProt ID: Q99460
• NCBI: NP_002798.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Rpn2; 26S proteasome subunit p112; P112; S1; 26S p
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PSMD1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PSMD1 antibody (orb1524061) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of mouse PSMD1 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PSMD1 antibody (orb1524061) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human PSMD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (200 µg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSMD1 antibody (orb1524061) used for IP at 6 µg per reaction. PSMD1 was also immunoprecipitated by a previous lot of this antibody (orb1524061) and a second antibody against a different epitope of PSMD1.

Detection of mouse PSMD1 by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PSMD1 (orb1524061) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human PSMD1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PSMD1 (orb1524061) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB