PSMC4 antibody

• Catalog Number: orb340842
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PSMC4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human PSMC4
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PSMC4
• Entrez: 5704, 23996, 117262
• UniProt ID: P43686, P54775, Q63570
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: MIP224; TBP7; 26S protease regulatory subunit 6B;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PSMC4 expression in HepG2 (A), A549 (B), Hela (C), Jurkat (D), mouse liver (E) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunofluorescent analysis of PSMC4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.