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Catalog Number | orb1265076 |
---|---|
Category | Antibodies |
Description | PSMC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit Ig |
Immunogen | This PRS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human PRS4. |
Concentration | batch dependent |
Dilution range | For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 49 kDa |
Target | PSMC1 |
UniProt ID | P62191 |
NCBI | P62191 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | 26S protease regulatory subunit 4, P26s4, 26S prot Read more... |
Note | For research use only |
Application notes | For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with PRS4 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with PRS4 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis in A549, Hela cell line and mouse heart and lung, rat lung tissue lysates (35 ug/lane).
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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