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Catalog Number | orb2292322 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant PSMA1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1D9-1C7 |
Tested applications | ELISA, IF, WB |
Reactivity | Human |
Isotype | IgG1 kappa |
Immunogen | PSMA1 (AAH02577, 1 a.a. ~ 263 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH |
NCBI | AAH02577 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence of monoclonal antibody to PSMA1 on HeLa cell. [antibody concentration 60 ug/ml]
PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Western Blot analysis of PSMA1 expression in HeLa.
Western Blot analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody (M01), clone 1D9-1C7. Lane 1: PSMA1 transfected lysate (30.2 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody (M01), clone 1D9-1C7 (Cat # orb2292322). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (54.67 KDa).