PSCA antibody

• Catalog Number: orb20074
• Category: Antibodies
• Description: Goat polyclonal to PSCA
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA
• Reactivity: Human
• Dilution range: ELISA: 1:1000, WB: 1 μg/ml
• Conjugation: Unconjugated
• MW: 12.9
• Target: PSCA (aa27-40)
• Entrez: 8000
• Protein Sequence: KAQVSNEDCLQVEN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PSCA antibody, anti prostate stem cell antige
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments in Human Prostate lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Expiration Date: 12 months from date of receipt.

orb20074 Flow cytometric analysis of paraformaldehyde fixed LNCaP cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb20074 Immunofluorescence analysis of paraformaldehyde fixed LNCaP cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).