PSA Antibody

SKU: orb385656

Description

Prostate specific antigen / PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. Using a Prostate specific antigen antibody, the protein can be detected in the sera of patients with prostatic carcinoma and expression level correlates positively with the likelyhood of cancer.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE ): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KLK3/801
• Immunogen: Full length human recombinant protein was used as the immunogen for this PSA antibody.
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE Analysis of Purified, BSA-Free PSA Antibody (KLK3/801). Confirmation of Integrity and Purity of the Antibody.

PSA antibody IHC testing of formalin-paraffin human prostate carcinoma.