PSA Mouse Monoclonal Antibody

SKU: orb11310

Description

PSA Mouse Monoclonal Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: BRC5
• Immunogen: Full length native protein purified from human full length
• Target: KLK3
• Molecular Weight: 23 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Concentration: ≥1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APS; KLK2A1; PSA; hK3; KLK3_HUMAN; KLK3; Gamma-seminoprotein (Seminin); Kallikrein-3; P-30 antigen; Semenogelase; 3.4.21.77; kallikrein related peptidase 3; kallikrein 3, (prostate specific antigen); prostate specific antigen

Tissue/Cell: human prostate carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PSA (Pa3) Polyclonal Antibody, Unconjugated (orb11310) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

KLK3

UniProt

P07288