PSA (Pa3) Mouse Monoclonal Antibody

• Catalog Number: orb11310
• Category: Antibodies
• Description: PSA (Pa3) Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Pa3
• Tested applications: ELISA, IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: Full length native protein purified from human full length
• Antibody Type: Primary Antibody
• Concentration: ≥1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: KLK3
• UniProt ID: P07288
• RRID: AB_10751651
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Alternative names: antigen, prostate-specific; APS; gamma seminoprote
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: human prostate carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PSA (Pa3) Polyclonal Antibody, Unconjugated (orb11310) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.